Novel Promoter Strategy Enhances Safety and Efficiency of CAR-T Therapy in Acute B Cell Leukemia
Beijing, Tuam Tshoj - Lub Xya Hli 23, 2024- Hauv kev tsim kho tshiab, Lu Daopei Tsev Kho Mob, koom tes nrog Hebei Senlang Biotechnology, tau nthuav tawm cov txiaj ntsig tau zoo los ntawm lawv txoj kev tshawb fawb tshiab ntawm chimeric antigen receptor T (CAR-T) cell therapy. Txoj kev tshawb no, uas tsom mus rau kev ua tau zoo thiab kev nyab xeeb ntawm CAR-T hlwb tsim nrog cov neeg txhawb nqa sib txawv, qhia txog kev nce qib tseem ceeb hauv kev kho mob rov qab los yog rov ua dua tshiab ntawm B cell leukemia (B-ALL).
Txoj kev tshawb no, hu ua "Promoter Usage Regulating Surface Density of CAR Molecules May Modulate the Kinetics of CAR-T Cells In Vivo," tshawb nrhiav seb qhov kev xaiv ntawm cov neeg txhawb nqa tuaj yeem cuam tshuam kev ua haujlwm ntawm CAR-T hlwb. Cov kws tshawb fawb Jin-Yuan Ho, Lin Wang, Ying Liu, Min Ba, Junfang Yang, Xian Zhang, Dandan Chen, Peihua Lu, thiab Jianqiang Li los ntawm Hebei Senlang Biotechnology thiab Lu Daopei Tsev Kho Mob spearheaded kev tshawb fawb no.
Lawv qhov kev tshawb pom qhia tias siv MND (myeloproliferative sarcoma virus MPSV enhancer, tsis zoo tswj cheeb tsam NCR tshem tawm, d1587rev primer binding site hloov) txhawb nqa hauv CAR-T hlwb ua rau qis qhov ntom ntom ntawm CAR molecules, uas nyob rau hauv lem txo cytokine ntau lawm. Qhov no yog qhov tseem ceeb vim tias nws pab txo qis cov kev mob tshwm sim hnyav feem ntau cuam tshuam nrog CAR-T txoj kev kho, xws li cytokine release syndrome (CRS) thiab CAR-T cell-related encephalopathy syndrome (CRES).
Qhov kev sim tshuaj ntsuam xyuas, sau npe hauv ClinicalTrials.gov tus cim NCT03840317, suav nrog 14 tus neeg mob muab faib ua ob pawg: ib qho tau txais MND-tsav CAR-T hlwb thiab lwm qhov tau txais EF1A txhawb nqa-tsav CAR-T hlwb. Zoo kawg nkaus, txhua tus neeg mob tau kho nrog MND-tsav CAR-T hlwb tau ua tiav kev tshem tawm, nrog rau feem ntau ntawm lawv qhia cov kab mob me me-tsis zoo tom qab thawj lub hlis. Txoj kev tshawb no kuj tau tshaj tawm qhov tshwm sim tsawg dua ntawm CRS thiab CRES nyob rau hauv cov neeg mob kho nrog MND-tsav CAR-T hlwb piv rau cov kho nrog EF1A-tsav hlwb.
Dr. Peihua Lu los ntawm Lu Daopei Tsev Kho Mob tau qhia qhov kev cia siab txog qhov muaj peev xwm ntawm txoj hauv kev tshiab no, hais tias, "Peb txoj kev koom tes nrog Hebei Senlang Biotechnology tau ua rau muaj kev nkag siab tseem ceeb rau kev kho CAR-T xov tooj ntawm tes. ntawm kev kho mob thaum tuav nws txoj kev ua tau zoo Qhov no yog ib kauj ruam tseem ceeb hauv kev ua CAR-T txoj kev kho kom yooj yim dua thiab zam tau rau cov neeg mob. "
Txoj kev tshawb no tau txais kev txhawb nqa los ntawm cov nyiaj pab los ntawm Natural Science Foundation ntawm Hebei Xeev thiab Lub Tsev Haujlwm Saib Xyuas Kev Tshawb Fawb thiab Tshuab ntawm Hebei Xeev. Nws qhia txog qhov tseem ceeb ntawm kev xaiv tus neeg txhawb nqa hauv kev txhim kho CAR-T cell kho thiab qhib txoj hauv kev tshiab rau kev kho mob qog noj ntshav kom nyab xeeb thiab zoo dua.